Carcinoembryonic antigen: characterization of binding with insoluble lectins
نویسندگان
چکیده
منابع مشابه
Characterization of poliovirus replicons encoding carcinoembryonic antigen.
Recombinant vaccines hold great promise for the prevention and therapy of infections diseases and cancer. We have explored the use of poliovirus as a recombinant vector to deliver genes into cells for the purpose of vaccination. For our studies, we have chosen to express the gene-encoding carcinoembryonic antigen (CEA) using a novel poliovirus vector. We have constructed a recombinant CEA-polio...
متن کاملRadioimmunoelectrophoretic binding assay for the detection of carcinoembryonic antigen.
A new radioimmunoassay (RIA) method has been de scribed. This radioimmunoelectrophoretic binding (REB) technique has been applied to the carcinoembryonic antigen system. In contrast to the RIA method with the use of perchloric acid extraction, in the REB technique the require ment for lengthy and possibly biologically degrading pro cedures on relatively large volumes of sera has been eliminated...
متن کاملSite of carcinoembryonic antigen binding to Kupffer cells.
the stromal-vascular fraction separated from the adipocytes by centrifugation. T h e stromal-vascular cells were seeded ( 2 0 0 0 0 0 cells/ml; 2 m1/25 cm? tissue culture flask) in Medium 199 supplemented with 200h (v/v) fetal calf serum and incubated at 37°C. T h e adipocyte fraction from the digests was also collected and washed before being added ( I ml of cells/25 cm? tissue culture flask) ...
متن کاملSelective binding of carcinoembryonic antigen using imprinted polymeric hydrogels.
A poly(allylamine hydrochloride) carcinoembryonic antigen-imprinted hydrogel was synthesized using a water-soluble crosslinker, ethylene glycol diglycidyl ether, to investigate its viability for protein recognition. The imprinting factor of the imprinted hydrogel toward carcinoembryonic antigen was found to be approximately 5, while the imprinting factor of the imprinted hydrogel toward alpha-f...
متن کاملThe binding of carcinoembryonic antigen by antibody and its fragments.
In order to assess the potency of antigenic fragments of carcinoembryonic antigen (CEA) in the radioimmune assay; it is necessary to know whether the high affinity of goat anti-CEA antibody (which makes possible the detection of as little as 10--11M CEA) is due to bivalent binding of the CEA molecule. Immunoglobulin G and the F(ab')2 and Fab fragments derived from it were prepared from an anti-...
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ژورنال
عنوان ژورنال: British Journal of Cancer
سال: 1975
ISSN: 0007-0920,1532-1827
DOI: 10.1038/bjc.1975.63